Immunological and genetic predictors of breast cancer

Aim. To investigate the associations of idiotypic IgA antibodies against benzo[a]pyrene, estradiol and progesterone (IgA 1 -Bp, IgA1 -E2, - Pg) with corresponding anti-idiotypic IgG to (IgG 2 -E2 -Pg) gene polymorphisms CYP1A1, CYP1A2, CYP1B1, CYP17A1, CYP19A1, GSTM1, GSTT1, GSTP1 in patients stage breast cancer. Materials Methods . Idiotypic serum 240 healthy women 505 cancer were measured by enzyme-linked immunosorbent assay. Prevalence CYP1A1 (rs4646903), CYP1A2 (rs762551), CYP1B1 (rs1056836), CYP19A1 (rs2470152), GSTM1(del), GSTT1(del), (rs1695) 530 694 determined real-time polymerase chain reaction. Result s. Low personal -Bp/IgA -Pg < -E2/IgA ratios combination low ≤ 4 high > levels found 20.6% 4.5% (p 0.0001; OR = 0.2). -Bp/IgA1 Pg revealed 7.4% 2.8% 0.009; 0.4). These two variants integrated marked as protective immunological phenotype. High Bp/IgA combined or 17.2% and27.2% 0.006; 1.8) 6.4% 18.3% 3.3), correspondingly. pro-carcinogenic only estrogen receptor-positive (ER+) polymorphism was associated exclusively receptor-negative (ER-) 0.004; 1.56). No interrelations be tween phenotypes studied CYP GST genes have been found. Conclusion Pro-carcinogenic phenotype rs1695 within independent predictors ER+ ER-